Oxysterol Binding Protein-Like 1A (OSBPL1A) antibody

Antigen: Oxysterol Binding Protein-Like 1A (OSBPL1A)

Synonyms: Gm753, Osbpl1b, G430090F17Rik, ORP1, ORP-1, OSBPL1B, FLJ10217, Osbpl1a, zgc:55948, wu:fi36a04, si:dkey-121a11.8, DKFZp459N1538

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185115

Additional Information

Alternative name: ORP1

Gene ID: 114876, 64291 (mouse), 259221 (rat)

Immunogen: C-YWDRNYFNLPDIY

Format: Affinity-purified

Description: All names and symbols: OSBPL1A, ORP1, OSBPL1B, FLJ10217, oxysterol binding protein-like 1A, OSBP-related protein 1, oxysterol-binding protein-like 1B, oxysterol-binding protein-related protein 1. Official Symbol: OSBPL1A. RefSeq number(s): NP_060500, NP_542164, NP_579802

Specificity: This antibody is expected to recognise all three human isoforms of this protein (represented by NP_060500, NP_542164, NP_579802). Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 48kDa band observed in Human Skeletal Muscle lysates (predicted MW of 50kDa according to NP_060500). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Images

ABIN185115 staining (0.5µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Lehto, Laitinen, Chinetti et al.: "The OSBP-related protein family in humans." in: Journal of lipid research, Vol. 42, Issue 8, pp. 1203-13, 2001 (PubMed).