Pleckstrin (PLEK) antibody

Antigen: Pleckstrin (PLEK)

Synonyms: P47, FLJ27168, 2010300B13Rik, MGC69065, PLEK

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185127

Additional Information

Alternative name: Pleckstrin

Gene ID: 5341

Immunogen: C-EWIKAIQMASRTGK

Format: Affinity-purified

Description: All names and symbols: pleckstrin, PLEK, P47, p47. Official Symbol: PLEK. RefSeq number(s): NP_002655. Gene ontology terms: calcium ion binding

Specificity: Human 350 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 45kDa band observed in Human Heart and PBMC lysates (predicted MW of 40kDa according to NP_002655). Recommended for use at 0.05-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185127 staining (0.05µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tyers, Rachubinski, Stewart et al.: "Molecular cloning and expression of the major protein kinase C substrate of platelets." in: Nature, Vol. 333, Issue 6172, pp. 470-3, 1988 (PubMed).