Polymerase (DNA Directed), lambda (POLL) antibody

Antigen: Polymerase (DNA Directed), lambda (POLL)

Synonyms: AV007317, 1110003P06Rik, T10O24.13, T10O24_13, BETAN, FLJ46002, POLKAPPA, zgc:66320, zgc:77901, wu:fe49a04, POLL, beta-n, pol-kappa

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185143

Additional Information

Alternative name: POLL / BETA-N

Gene ID: 27343, 56626 (mouse)

Immunogen: C-LGLPYREPAERDW

Format: Affinity-purified

Description: All names and symbols: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N). Official Symbol: POLL. RefSeq number(s): NP_037406. Gene ontology terms: DNA binding, beta DNA polymerase activity, lambda DNA polymerase activity, lyase activity, manganese ion binding, metal ion binding, transferase activity, DNA repair, DNA replication, nucleotide-excision repair, somatic hypermutation of antibody genes

Specificity: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region. Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1µg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185143 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Garcia-Diaz, Bebenek, Sabariegos et al.: "DNA polymerase lambda, a novel DNA repair enzyme in human cells." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 13184-91, 2002 (PubMed).