MPP5 antibody (N-Term)
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- Target See all MPP5 Antibodies
- MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This MPP5 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- PALS / MPP5
- Sequence
- NKASPPFPLI SNAQ
- Cross-Reactivity
- Cow, Dog, Human, Pig
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- NKASPPFPLISNAQ-C
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Preliminary testing showed a band at approx 75 kDa in Human Cerebellum lysate after 1 μg/mL antibody staining (calculated MW of 73.4 kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))
- Alternative Name
- MPP5 (MPP5 Products)
- Synonyms
- PALS1 antibody, 3830420B02Rik antibody, AI255216 antibody, AI644496 antibody, Pals1 antibody, mpp5 antibody, nok antibody, MAGUK p55 subfamily member 5 antibody, membrane palmitoylated protein 5 antibody, membrane protein, palmitoylated 5 L homeolog antibody, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) antibody, membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) antibody, Tsp_03061 antibody, MPP5 antibody, mpp5.L antibody, Mpp5 antibody, mpp5a antibody
- Background
- MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5, membrane protein, palmitoylated 5, stardust
- Gene ID
- 64398
- NCBI Accession
- NP_071919
- Pathways
- Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization
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