Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5) antibody

Antigen: Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5)

Synonyms: PALS1, FLJ12615

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185150

Additional Information

Alternative name: PALS / MPP5

Gene ID: 64398

Immunogen: NKASPPFPLISNAQ-C

Format: Affinity-purified

Description: All names and symbols: MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5. Official Symbol: MPP5. RefSeq number(s): NP_071919

Specificity: Human 503 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75kDa band observed in Human Brain lysate (calculated MW of 77.3kDa according to NP_071919). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows strong alternating staning of muscle fibres in longitudinal section. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185150 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185150 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Kamberov, Makarova, Roh et al.: "Molecular cloning and characterization of Pals, proteins associated with mLin-7." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11425-31, 2000 (PubMed).