Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6) antibody

Antigen: Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6)

Synonyms: VAM1, p55T, PALS2, VAM-1, P55t, Pals2, MGC189400, mpp6, MGC55662, zgc:55662

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185151

Additional Information

Alternative name: PALS2 / VAM1 / MPP6

Gene ID: 51678, 56524 (mouse)

Immunogen: QQVLENLTELPSS-C

Format: Affinity-purified

Description: All names and symbols: MPP6, VAM1, p55T, VAM-1, membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6), MAGUK protein p55T, VELI-associated MAGUK 1, protein associated with Lin7 2. Official Symbol: MPP6. RefSeq number(s): NP_057531.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in lysates of cell line HEK293 (calculated MW of 61.1kDa according to NP_057531.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185151 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kamberov, Makarova, Roh et al.: "Molecular cloning and characterization of Pals, proteins associated with mLin-7." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11425-31, 2000 (PubMed).