PARD6A antibody (C-Term)

Catalog No. ABIN185152

This Goat Polyclonal antibody specifically detects PARD6A in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for PARD6A antibody (C-Term) (ABIN185152)

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARD6A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PARD6A Antibody

Binding Specificity: C-Term

Purpose: PAR6alpha / PARD6A

Sequence: GSRIRGDGSG FSL

Specificity: This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocar

Western Blot: Approx 40 kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4 kDa according to NP_058644.1) . Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PARD6A

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Alternative Name: PARD6A

Background: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning d

Gene ID: 50855

NCBI Accession: NP_058644, NP_001032358

Pathways: Cell-Cell Junction Organization