PDZ and LIM Domain 1 (PDLIM1) antibody

Antigen: PDZ and LIM Domain 1 (PDLIM1)

Synonyms: CLIM1, CLP36, CLP-36, hCLIM1, pdlim1, MGC76041, MGC64465, PDLIM1, clim1, wu:fa19a03, wu:fy34f10, zgc:110681, Clim1, mClim1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185153

Additional Information

Alternative name: CLIM1 / PDLIM1

Gene ID: 9124

Immunogen: C-TPPEGYEVVTVFPK

Format: Affinity-purified

Description: All names and symbols: PDLIM1, CLIM1, CLP36, ELFIN, CLP-36, hCLIM1, PDZ and LIM domain 1 (elfin), carboxy terminal LIM domain protein 1. Official Symbol: PDLIM1. RefSeq number(s): NP_066272. Gene ontology terms: response to oxidative stress

Specificity: Human 329 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 37kDa band observed in human lung and heart lysates (predicted MW of 38kDa according to NP_066272). Recommended for use at 0.1-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185153 staining (0.1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kotaka, Kostin, Ngai et al.: "Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2." in: Journal of cellular biochemistry, Vol. 78, Issue 4, pp. 558-65, 2000 (PubMed).