Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) antibody

Antigen: Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2)

Synonyms: RCH2, SRP1, IPOA5, NPI-1, QIP2, RCH1, IPOA1, SRP1alpha, Gm1055, AW146299, KPNA1, DKFZp459M124, ima2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN185157

Additional Information

Alternative name: KPNA2 / IPOA1

Gene ID: 3838, 16647 (mouse), 85245 (rat)

Immunogen: C-QVQDGAPGTFNF

Format: Affinity-purified

Description: All names and symbols: KPNA2, IPOA1 QIP2, RCH1, SRP1alpha, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), RAG cohort 1, importin alpha 1. Official Symbol: KPNA2. RefSeq number(s): NP_002257.1. Gene ontology terms: nuclear localization sequence binding, protein binding, regulation of DNA recombination, G2 phase of mitotic cell cycle, spindle pole body and microtubule cycle (sensu Saccharomyces), NLS-bearing substrate-nucleus import, DNA metabolism, importin, alpha-subunit, nucleoplasm, cytoplasm, nucleus

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 55-60kDa band observed in Hela lysates (predicted size of approx. 58kDa according to NP_002257.1). Recommended for use at 0.1-0.5µg/ml Immunofluoresence: in 4% formaldahyde-fixed HeLa staining is mostly cytoplasmic, with some nuclear staining and clear nuclear pore staining.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185157 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185157 staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.

Publications

Publications: Cuomo, Kirch, Gyuris et al.: "Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 13, pp. 6156-60, 1994 (PubMed).