Vacuolar Protein Sorting 29 Homolog (S. Cerevisiae) (VPS29) (C-Term) antibody

Antigen: Vacuolar Protein Sorting 29 Homolog (S. Cerevisiae) (VPS29)

Synonyms: VPS29, fb06g05, MGC56191, MGC86638, zgc:56191, zgc:86638, wu:fb06g05, PEP11, VPT6, DC7, DC15, FLJ20492, DKFZp564F0223, AW049835, 2010015D08Rik, MGC127483, vps29, ATVPS29, MAG1, MAIGO 1, VACUOLAR PROTEIN SORTING 29, 17.m07782

Binding Site: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185173

Additional Information

Alternative name: VPS29

Gene ID: 51699, 56433 (mouse)

Immunogen: C-DDVKVERIEYKKP

Description: All names and symbols: VPS29, DC7, DC15, PEP11, FLJ20492, DKFZp564F0223, vacuolar protein sorting 29 (yeast), x 007 protein, retromer protein, vacuolar sorting protein VPS29/PEP11, vacuolar protein sorting 29 (yeast homolog). Official Symbol: VPS29. RefSeq number(s): NP_057310.1, NP_476528.1

Specificity: This antibody is expected to recognise both human isoforms (represented by NP_057310.1, NP_476528.1). Human, Mouse, Rat, Dog

Synonyms: VPS29, fb06g05, MGC56191, MGC86638, zgc:56191, zgc:86638, wu:fb06g05, PEP11, VPT6, DC7, DC15, FLJ20492, DKFZp564F0223, AW049835, 2010015D08Rik, MGC127483, vps29, ATVPS29, MAG1, MAIGO 1, VACUOLAR PROTEIN SORTING 29, 17.m07782

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 20+21kDa bands observed in Human Spleen lysates (calculated MW of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1). Recommended concentration: 0.1-0.3µg

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185173 staining (0.1 ug/ml) of Human Spleen lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Haft, de la Luz Sierra, Bafford et al.: "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes." in: Molecular biology of the cell, Vol. 11, Issue 12, pp. 4105-16, 2000 (PubMed).