Serine/threonine Kinase 35 (STK35) antibody

Antigen: Serine/threonine Kinase 35 (STK35)

Synonyms: CLIK1, STK35L1, Stk35l1, 1700054C12Rik, clik1, stk35l1, MGC69346, MGC153287, im:6905214, zgc:153287, MGC115406

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185178

Additional Information

Alternative name: Clik1 / STK35

Gene ID: 140901, 67333 (mouse)

Immunogen: Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2

Format: Affinity-purified

Description: All names and symbols: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form. Official Symbol: STK35. RefSeq number(s): NP_543026.2.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Vallenius, Maekelae: "Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein." in: Journal of cell science, Vol. 115, Issue Pt 10, pp. 2067-73, 2002 (PubMed).

Goyal, Behring, Kumar et al.: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, 2009 (PubMed).