Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) antibody

Antigen: Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35)

Synonyms: Mem3, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, VPS35, MGC128544, 23.m05999, DKFZp459E1510, 7B4, CD144, FLJ17376, CG5625, DmelCG5625, Dvps35, vps35, AI647796, MGC81959, bZ34G2.3, MGC136268, MGC162844, wu:fa66e03, wu:fb95c08, zgc:136268, si:rp71-34g2.3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185182

Additional Information

Alternative name: VPS35 / MEM3

Gene ID: 55737, 65114 (mouse)

Immunogen: C-SPESEGPIYEGLIL

Description: All names and symbols: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3. Official Symbol: VPS35. RefSeq number(s): NP_060676.2

Specificity: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical. Human, Mouse, Rat

Synonyms: Mem3, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, VPS35, MGC128544, 23.m05999, DKFZp459E1510, 7B4, CD144, FLJ17376, CG5625, DmelCG5625, Dvps35, vps35, AI647796, MGC81959, bZ34G2.3, MGC136268, MGC162844, wu:fa66e03, wu:fb95c08, zgc:136268, si:rp71-34g2.3

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185182 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185182 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3 ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.

Publications

Zhang, Yu, Gao et al.: "Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21." in: Genomics, Vol. 70, Issue 2, pp. 253-7, 2001 (PubMed).