SH3-Domain Binding Protein 1 (SH3BP1) antibody

Antigen: SH3-Domain Binding Protein 1 (SH3BP1)

Synonyms: 3BP-1, SH3BP1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185183

Additional Information

Alternative name: SH3BP1

Gene ID: 23616

Immunogen: C-PQPRPRSLASETN

Format: Affinity-purified

Description: All names and symbols: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981. Official Symbol: SH3BP1. RefSeq number(s): NP_061830.3

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Lung and Kidney lysates (predicted MW of 71kDa according to NP_061830). Recommended for use at 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185183 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185183 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Publications

Publications: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).