SIRT4 antibody (C-Term)

Catalog No. ABIN185191

This Goat Polyclonal antibody specifically detects SIRT4 in WB, IHC and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for SIRT4 antibody (C-Term) (ABIN185191)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SIRT4 Antibody

Binding Specificity: C-Term

Purpose: SIRT4

Sequence: SRCGELLPLI DPC

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SRCGELLPLIDPC, from the C Terminus of the protein sequence according to NP_036372.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx. 38 kDa band observed in Human Kidney lysates (calculated MW of 35.2 kDa according to NP_036372.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SIRT4 antibody (ABIN185191)

do Amaral, Ueno, Oliveira, Borsonello, Vanzela, Ribeiro, Alves, Barbosa, Carneiro, Boschero: "Reduced expression of SIRT1 is associated with diminished glucose-induced insulin secretion in islets from calorie-restricted rats." in: The Journal of nutritional biochemistry, Vol. 22, Issue 6, pp. 554-9, (2011) (PubMed).

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4

Background: SIRT4, SIR2L4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae), MGC130046, MGC130047, MGC57437, SIR2L4, sirtuin 4, sir2-like 4, sirtuin 4, sirtu

Gene ID: 23409, 75387

NCBI Accession: NP_036372

Pathways: Negative Regulation of Hormone Secretion