Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) antibody

Antigen: Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A)

Synonyms: CPK, MGC142218, PI3-K-C2A, DKFZp686L193, PI3-K-C2(ALPHA), PIK3C2A, cpk, pi3-k-c2a, DKFZp469H1410

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185194

Additional Information

Alternative name: PIK3C2A

Gene ID: 5286, 18704 (mouse)

Immunogen: C-TVKWYQLTAATYL

Description: All names and symbols: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase. Official Symbol: PIK3C2A. RefSeq number(s): NP_002636. Gene ontology terms: 1-phosphatidylinositol 3-kinase, phosphatidylinositol biosynthesis

Specificity: Human, Mouse 1686 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185194 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Molz, Chen, Hirano et al.: "Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13892-9, 1996 (PubMed).