Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1) antibody

Antigen: Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1)

Synonyms: FDG, UNG3, HMUDG, MGC104370, SMUG1, C85220, 1200013B09Rik, A930006H09Rik, MGC84842, xsmug1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185204

Additional Information

Alternative name: SMUG1

Gene ID: 23583

Immunogen: PQAFLLGSIHEPA-C

Format: Affinity-purified

Description: All names and symbols: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3. Official Symbol: SMUG1. RefSeq number(s): NP_055126. Gene ontology terms: uracil DNA N-glycosylase activity, single-stranded DNA binding activity, DNA repair

Specificity: Human 270 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 35kDa band observed in Molt-4 lysates (predicted MW of 31kDa according to NP_055126. Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN185204 staining (0.5µg/ml) of Molt-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Boorstein, Cummings, Marenstein et al.: "Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1." in: The Journal of biological chemistry, Vol. 276, Issue 45, pp. 41991-7, 2001 (PubMed).