Paired Box 3 (PAX3) antibody

Antigen: Paired Box 3 (PAX3)

Synonyms: WS1, CDHS, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, pax3, zgc:92547, PAX3, ws1, cdhs, hup2, pax-3, xpax3, MGC89554, MGC131061, Sp, Pax-3, splotch

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185216

Additional Information

Alternative name: PAX3

Gene ID: 5077, 18505 (mouse), 114502 (rat)

Immunogen: TTLAGAVPRMM-C

Format: Affinity-purified

Description: All names and symbols: PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1). Official Symbol: PAX3. RefSeq number(s): NP_000429.2, NP_039230.1, NP_852122,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1. Gene ontology terms: transcription activating factor, transcription factor activity, hearing, histogenesis and organogenesis, neurogenesis, apoptosis, oncogenesis, transcription from Pol II promoter

Specificity: This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2, NP_039230.1, NP_852122,.1 NP_852123.1, NP_852124.1, NP_852125.1 and NP_852126.1). Human, Mouse, Rat 215 aa, 206 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 30-35kDa band in Human Kidney lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2, NP_039230.1, NP_852122,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Organogenesis, Lineage Markers, Transcription Factors

Restrictions: For Research Use only

Images

ABIN185216 (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Tsukamoto, Nakamura, Niikawa: "Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues." in: Human genetics, Vol. 93, Issue 3, pp. 270-4, 1994 (PubMed).