Regulator of G-Protein Signaling 14 (RGS14) antibody

Antigen: Regulator of G-Protein Signaling 14 (RGS14)

Synonyms: MGC108631, MGC80558, RGS14, rgs14

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185233

Additional Information

Alternative name: RGS14

Gene ID: 10636

Immunogen: C-IGGSLNSTTDSAL

Format: Affinity-purified

Description: All names and symbols: RGS14, regulator of G-protein signalling 14, regulation of G protein signaling 14. Official Symbol: RGS14. RefSeq number(s): NP_006471. Gene ontology terms: GTPase activator activity, receptor signaling protein activity, regulation of G-protein coupled receptor protein signaling pathway, signal transduction

Specificity: Human 566 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:16,000. Western Blot: Approx 65-70kDa band observed in Jurkat lysates (predicted MW of 65kDa according to NP_006471). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Immunology

Restrictions: For Research Use only

Images

ABIN185233 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Snow, Antonio, Suggs et al.: "Molecular cloning and expression analysis of rat Rgs12 and Rgs14." in: Biochemical and biophysical research communications, Vol. 233, Issue 3, pp. 770-7, 1997 (PubMed).