XAGE1B/GAGED2 antibody (C-Term)

Catalog No. ABIN185262

This anti-XAGE1B/GAGED2 antibody is a Goat Polyclonal antibody detecting XAGE1B/GAGED2 in ELISA and IHC. Suitable for Human.

Quick Overview for XAGE1B/GAGED2 antibody (C-Term) (ABIN185262)

Target: XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This XAGE1B/GAGED2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-XAGE1B/GAGED2 Antibody

Binding Specificity: C-Term

Purpose: XAGE1 (Isoform 1d) / GAGED2

Sequence: CGFGFRRQGE DNT

Specificity: This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1, NP_001091067.1, NP_001091074.1, NP_001091062.1, NP_001091065.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CGFGFRRQGEDNT

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6 μg/mL.

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0 kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the produ

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for XAGE1B/GAGED2

Target: XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))

Alternative Name: GAGED2

Background: GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAGE-1 protein

Gene ID: 9503

NCBI Accession: NP_597673