Pirin antibody (N-Term)

Catalog No. ABIN185265

This anti-Pirin antibody is a Goat Polyclonal antibody detecting Pirin in ELISA, WB and IHC. Suitable for Human.

Quick Overview for Pirin antibody (N-Term) (ABIN185265)

Target: Pirin (PIR)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Pirin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Pirin Antibody

Binding Specificity: N-Term

Purpose: Pirin

Sequence: GSSKKVTLSV LSREC

Specificity: Variants (NP_003653.1, NP_001018119.1) encode the same protein

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GSSKKVTLSVLSREC, from the N Terminus of the protein sequence according to NP_003653.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 33 kDa band observed in Human Heart lysate (calculated MW of 32.1 kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Pirin

Target: Pirin (PIR)

Alternative Name: PIR

Background: PIR, pirin, pirin (iron-binding nuclear protein)

Gene ID: 8544

NCBI Accession: NP_003653