Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Antigen: Baculoviral IAP Repeat-Containing 5 (BIRC5)

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185270

Additional Information

Alternative name: Survivin / BIRC5

Gene ID: 332

Immunogen: CVRRAIEQLAAMD

Format: Affinity-purified

Description: All names and symbols: BIRC5, survivin, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), apoptosis inhibitor 4. Official Symbol: BIRC5. RefSeq number(s): NP_001159.2, NP_001012271.1. Gene ontology terms: 3-oxo-5-alpha-steroid 4-dehydrogenase activity, DNA repair, androgen metabolism, cell-cell signaling, electron transporter activity, integral to membrane, male gonad development, methylated-DNA-[protein]-cysteine S-methyltransferase activity, microsome, oxidoreductase activity, sex determination, sex differentiation

Specificity: This antibody is expected to recognize the reported isoforms 1 and 3 (NP_001159.2 and NP_001012271.1 resp.) Human

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185270 (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Li, Ambrosini, Chu et al.: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).