TAO Kinase 3 (TAOK3) antibody

Antigen: TAO Kinase 3 (TAOK3)

Synonyms: DPK, JIK, MAP3K18, FLJ31808, DKFZp666H245, A130052D22, 2900006A08Rik, A430105I05Rik, TAOK3, DKFZp459L243

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185273

Additional Information

Alternative name: JIK / TAOK3

Gene ID: 51347, 304530 (rat)

Immunogen: CHKKDHVFIRDEAGHGD

Format: Affinity-purified

Description: All names and symbols: TAOK3, JIK, TAO kinase 3, DPK, STE20-like kinase, STE2-like kinase. Official Symbol: TAOK3. RefSeq number(s): NP_057365. Gene ontology terms: ATP binding, MAPKKK cascade, autophosphorylation, negative regulation of JNK cascade, positive regulation of JNK cascade, protein serine/threonine kinase activity, transferase activity

Specificity: Human, Rat 898 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 100kDa band observed in human liver lysate (calculated MW of 105kDa according to NP_057365). Recommended concentration: 0.3-1.0 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185273 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Tassi, Biesova, Di Fiore et al.: "Human JIK, a novel member of the STE20 kinase family that inhibits JNK and is negatively regulated by epidermal growth factor." in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33287-95, 1999 (PubMed).