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Antibodies
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anti-Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) antibody
Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) antibody
| Antigen |
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| Synonyms |
DKK1, SK, DKK-1, MGC152787, dkk, dkk-1, xdkk1, Xdkk-1, Dickkopf1, dickkopf-1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (32), Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN185275 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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DKK1
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Gene ID
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22943
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Immunogen
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CDHHQASNSSRLHT
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Format
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Affinity-purified
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Description
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All names and symbols: DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis), dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1. Official Symbol: DKK1. RefSeq number(s): NP_036374. Gene ontology terms: Wnt receptor signaling pathway, development, extracellular, growth factor activity, negative regulation of Wnt receptor signaling pathway, signal transducer activity
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Specificity
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Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 38kDa band observed in human spleen and bone marrow lysates after 1µg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463). However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Embryogenesis, Wnt
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Restrictions
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For Research Use only
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Publications
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Fedi, Bafico, Nieto Soria et al.: "Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 19465-72, 1999 (PubMed).
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Alternatives for antigen "Dickkopf Homolog 1 (Xenopus Laevis) (DKK1)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (19), Goat (5), Sheep (1)
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Reactivities
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Human (53), Mouse (Murine) (22), Rat (Rattus) (22), Monkey (1)
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Applications
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Western Blotting (WB) (32), Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), N-Term (1)
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Alternatives 
