Peroxisome Proliferator-Activated Receptor delta (PPARD) (isoform 1) antibody

Antigen: Peroxisome Proliferator-Activated Receptor delta (PPARD)

Synonyms: NUC1, NUC-1, Nr1c2, Pparb, PPAR[b], Pparb/d, PPAR-beta, PPARdelta, PPAR-delta, FAAR, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPARBETA, ppard, pparb, PPARD

Epitope: isoform 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185282

Additional Information

Alternative name: PPAR delta (Isoform 1)

Gene ID: 5467

Swiss-Prot: NP_006229.1

Immunogen: Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine)

Format: Liquid

Description: This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus. Principal Names: OTTHUMP00000016257, OTTHUMP00000016256, peroxisome proliferator-activated receptor delta, nuclear hormone receptor 1, peroxisome proliferative activated receptor, delta, PPAR-beta, MGC3931, PPARB, NUCII, NR1C2, NUCI, NUC1, FAAR, PPARD. Official Symbol: PPARD

Specificity: This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus. Human, Mouse, Rat, Pig, Chicken 441 aa, 361 aa

Molecular Weight: 49.9kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kDa band observed in Human Lymphoblastoid and T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 1-3µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185282 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Park, Vogelstein, Kinzler: "Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 5, pp. 2598-603, 2001 (PubMed).