Four and A Half LIM Domains 1 (FHL1) antibody

Antigen: Four and A Half LIM Domains 1 (FHL1)

Synonyms: KYOT, FHL1B, FLH1A, SLIM1, XMPMA, SLIMMER, MGC111107, KyoT, MGC85575, fhl1, MGC89165, FHL1, MGC127148, DKFZp468O1927, fhl, zgc:92025

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185290

Additional Information

Alternative name: SLIMMER / FHL1 isoform B

Gene ID: 2273

Immunogen: CTVSRVSHPVSKARK

Format: Affinity-purified

Description: All names and symbols: FHL1, KYO-T, SLIM1, bA535K18.1, four and a half LIM domains 1, Four-and-a-half LIM domains 1, bA535K18.1 (LIM protein SLIMMER),. Official Symbol: FHL1. RefSeq number(s): AAC72886.1. Gene ontology terms: cell differentiation, cell growth, molecular_function unknown, muscle development, nucleus

Specificity: This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein. Human, Dog 323 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 30kDa band observed in Human Heart lysates is consistent with literature (PMID:10480922, calculated MW of 36.3kDa according to AAC72886). Recommended concentration: 0.01-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185290 (0.01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Brown, McGrath, Ooms et al.: "Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear" in: The Journal of biological chemistry, Vol. 274, Issue 38, pp. 27083-91, 1999 (PubMed).