Translocase of Inner Mitochondrial Membrane 50 Homolog (S. Cerevisiae) (TIMM50) antibody

Antigen: Translocase of Inner Mitochondrial Membrane 50 Homolog (S. Cerevisiae) (TIMM50)

Synonyms: TIM50, TIM50L, MGC102733

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185291

Additional Information

Alternative name: TIM50

Gene ID: 92609

Immunogen: CDVRTVLEHYALEDD

Format: Affinity-purified

Description: All names and symbols: TIMM50, TIM50L, translocase of inner mitochondrial membrane 50 homolog (yeast), Tim50-like protein, homolog of yeast Tim50. Official Symbol: TIMM50. RefSeq number(s): NP_001001563

Specificity: This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50. Human, Mouse, Rat, Dog 456 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approx 40kDa in Human Heart lysate after 0.5µg/ml antibody staining. This band corresponds to earlier findings in literature with different antibodies (PM

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185291 staining (0.5 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Guo, Cheong, Zhang et al.: "Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death." in: The Journal of biological chemistry, Vol. 279, Issue 23, pp. 24813-25, 2004 (PubMed).