Docking Protein 5 (DOK5) antibody

Antigen: Docking Protein 5 (DOK5)

Synonyms: MGC16926, C20orf180, 2700055C10Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185292

Additional Information

Alternative name: DOK5

Gene ID: 55816, 76829 (mouse)

Immunogen: CLHRTETFPAYRSEH

Format: Affinity-purified

Description: All names and symbols: DOK5, MGC16926, C20orf180, docking protein 5, downstream of tyrosine kinase 5, chromosome 20 open reading frame 180. Official Symbol: DOK5. RefSeq number(s): NP_060901, NP_808874. Gene ontology terms: insulin receptor binding

Specificity: This antibody is expected to recognise both reported isoforms of DOK5. Human, Mouse, Rat 306 aa, 198 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: A 26-28kDa band observed in human kidney, skeletal muscle and thymus lysates (calculated MW of 35.5kDa according to NP_060901). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

ABIN185292 (0.1µg/ml) staining of human kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185292 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Publications

Publications: Favre, Gerard, Clauzier et al.: "DOK4 and DOK5: new Dok-related genes expressed in human T cells." in: Genes and immunity, Vol. 4, Issue 1, pp. 40-5, 2003 (PubMed).

Cai, Dhe-Paganon, Melendez et al.: "Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25323-30, 2003 (PubMed).