Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) antibody

Antigen: Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3)

Synonyms: LXRA, LXR-a, RLD-1, NR1H3, LXR-alpha

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185294

Additional Information

Alternative name: LXR alpha / LXR beta

Gene ID: 10062, 74

Immunogen: CRLQDKKLPPLLSEI

Format: Affinity-purified

Description: All names and symbols: LXRA, LXR-a, RLD-1, NR1H3, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor. Official Symbol: NR1H3, NR1H2. RefSeq number(s): NP_005684, NP_009052. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, steroid hormone receptor activity, thyroid hormone receptor activity, transcription coactivator activity, transcription factor activity

Specificity: This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684) and to aa 443-456 of human LXR beta protein (NP_009052). Human, Mouse, Rat 447 aa, 461 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55kDa band observed in human spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Recommended concentration: 1-3µg/ml. Additional minor bands were also consistently observed at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185294 (1µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Joseph, McKilligin, Pei et al.: "Synthetic LXR ligand inhibits the development of atherosclerosis in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 11, pp. 7604-9, 2002 (PubMed).

Tangirala, Bischoff, Joseph et al.: "Identification of macrophage liver X receptors as inhibitors of atherosclerosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11896-901, 2002 (PubMed).