Retinoid X Receptor, gamma (RXRG) antibody

Antigen: Retinoid X Receptor, gamma (RXRG)

Synonyms: RXRC, NR2B3, RXRG, RXRgamma, xrxrg, RXR-gamma, MGC143016, rxrc, nr2b3, rxr, rxra, rxrg, etID309731.5, DKFZp468D1122, LOC100224780, LOC100231066, Nr2b3, MGC109416

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185297

Additional Information

Alternative name: RXR gamma

Gene ID: 6258

Immunogen: RQRSRERAESEAEC

Format: Affinity-purified

Description: All names and symbols: RXRG, RXRC, NR2B3, retinoid X receptor, gamma. Official Symbol: RXRG. RefSeq number(s): NP_008848. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, retinoid-X receptor activity, steroid binding, steroid hormone receptor activity, transcription factor activity

Specificity: This antibody is expected to recognise an epitope corresponding to aa 213-226 of human RXR gamma protein (hinge region) and does not cross-react with either RXR alpha or beta. Human, Mouse, Rat, Pig 463 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 48kDa band observed in human brain lysates (calculated MW of 50.9kDa according to NP_008848). Recommended concentration: 0.3-2µg/ml. Some bands of unknown identity were also consistently observed around 37 kDa. These bands were successfully blocked by incubation with the immunising peptide.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185297 (0.3µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Samad, Krezel, Chambon et al.: "Regulation of dopaminergic pathways by retinoids: activation of the D2 receptor promoter by members of the retinoic acid receptor-retinoid X receptor family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 26, pp. 14349-54, 1998 (PubMed).

Krezel, Ghyselinck, Samad et al.: "Impaired locomotion and dopamine signaling in retinoid receptor mutant mice." in: Science (New York, N.Y.), Vol. 279, Issue 5352, pp. 863-7, 1998 (PubMed).