ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4)

Synonyms: MRP4, MOATB, MOAT-B, EST170205, Mrp4, D630049P08Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Catalog no.: ABIN185302

Additional Information

Alternative name: ABCC4 / MRP4

Gene ID: 10257, 170924 (rat)

Swiss-Prot: NP_005836.2

Immunogen: Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Format: Liquid

Description: Principal Names: multidrug resistance-associated protein 4, multispecific organic anion transporter B, canalicular multispecific organic anion transporter (ABC superfamily), bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4), ATP-binding cassette, sub-family C, member 4, RP11-74A12.1, Multidrug Resistance Protein 4, canalicular multispecific organic anion transporter (ABC superfamily), ATP-binding cassette, sub-family C (CFTR/MRP), member 4, EST170205, MOAT-B, MOATB, MRP4, ABCC4. Official Symbol: ABCC4

Specificity: This antibody is expected to recognize isoform 1 (NP_005836.2) only. Human, Mouse, Rat 1325 aa

Molecular Weight: 149kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data obtained from a previous batch and kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3µg/ml. IHC: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4µg/ml. ICC/IF: This antibody (from a previous batch) has been successfully used in ICC/IF for human primary hepatocytes as described in the following paper: Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul,299(1):G126-35, PMID: 20395535.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185302 staining (0.5µg/ml) of rat renal cortex membranes (20µg total protein per lane). Detected by western blot using chemiluminescence. Data kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald.

ABIN185302 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Schuetz, Connelly, Sun et al.: "MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs." in: Nature medicine, Vol. 5, Issue 9, pp. 1048-51, 1999 (PubMed).

Jedlitschky, Tirschmann, Lubenow et al.: "The nucleotide transporter MRP4 (ABCC4) is highly expressed in human platelets and present in dense granules, indicating a role in mediator storage." in: Blood, Vol. 104, Issue 12, pp. 3603-10, 2004 (PubMed).