Target of Myb1-Like 2 (Chicken) (TOM1L2) antibody

Antigen: Target of Myb1-Like 2 (Chicken) (TOM1L2)

Synonyms: FLJ32746, MGC84791, MGC157155

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185312

Additional Information

Alternative name: TOM1L2

Gene ID: 146691

Immunogen: CRKKPERSEDALFAL

Format: Affinity-purified

Description: All names and symbols: TOM1L2, FLJ32746, target of myb1-like 2 (chicken), target of myb1 (chicken) homolog-like 1, target of myb1 (chicken) homolog-like 2. Official Symbol: TOM1L2. RefSeq number(s): NP_653279. Gene ontology terms: Golgi stack, intra-Golgi transport, intracellular protein transport, protein transporter activity

Specificity: Human, Mouse 240 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 60kDa band observed in Human Fibroblast A431 lysates (calculated MW of 51.8kDa according to NP_653279). Recommended concentration: 0.1-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185312 (0.03 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Blagoev, Ong, Kratchmarova et al.: "Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics." in: Nature biotechnology, Vol. 22, Issue 9, pp. 1139-45, 2004 (PubMed).