Prostaglandin Dehydrogenase 1 (HPGD) antibody

Antigen: Prostaglandin Dehydrogenase 1 (HPGD)

Synonyms: PGDH, PGDH1, 15-PGDH, SDR36C1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185316

Additional Information

Alternative name: Prostaglandin dehydrogenase 1

Gene ID: 3248

Immunogen: C-DYDTTPFQAKTQ

Format: Affinity-purified

Description: All names and symbols: Prostaglandin dehydrogenase 1, HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD). Official Symbol: HPGD. RefSeq number(s): NP_000851. Gene ontology terms: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity, electron transporter activity, metabolism, oxidoreductase activity, prostaglandin metabolism, prostaglandin-D synthase activity

Specificity: Human, Chimpanzee 266 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0kDa according to NP_000851. Recommended concentration: 0.1-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185316 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ensor, Yang, Okita et al.: "Cloning and sequence analysis of the cDNA for human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 14888-91, 1990 (PubMed).

Coggins, Latour, Nguyen et al.: "Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus." in: Nature medicine, Vol. 8, Issue 2, pp. 91-2, 2002 (PubMed).