Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2) antibody

Antigen: Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2)

Synonyms: S2, P97, Rpn1, TRAP2, MGC14274, Tex190, TEG-190, AA407121, 9430095H01Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185319

Additional Information

Alternative name: TRAP2 / Proteasome subunit 26S

Gene ID: 5708

Immunogen: C-VILRKNPNYDL

Format: Affinity-purified

Description: All names and symbols: PSMD2, S2, P97, TRAP2, MGC14274, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor . Official Symbol: PSMD2. RefSeq number(s): NP_002799. Gene ontology terms: proteasome regulatory particle (sensu Eukaryota), protein binding, regulation of cell cycle

Specificity: Human, Mouse, Rat, Chimpanzee 908 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 100kDa band observed in Human epidermoid carcinoma A431 lysates (calculated MW of 100kDa according to NP_002799). Recommended concentration: 0.03-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185319 (0.03 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kuai, Wooters, Hall et al.: "NAK is recruited to the TNFR1 complex in a TNFalpha-dependent manner and mediates the production of RANTES: identification of endogenous TNFR-interacting proteins by a proteomic approach." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53266-71, 2004 (PubMed).