PSMD2 antibody (C-Term)

Catalog No. ABIN185319

The Goat Polyclonal anti-PSMD2 antibody (ABIN185319) specifically detects PSMD2 in WB, ELISA, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PSMD2 antibody (C-Term) (ABIN185319)

Target: PSMD2 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PSMD2 Antibody

Binding Specificity: C-Term

Purpose: TRAP2 / Proteasome subunit 26S

Sequence: VILRKNPNYD L

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VILRKNPNYDL, from the C Terminus of the protein sequence according to NP_002799.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100 kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PSMD2

Target: PSMD2 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 2 (PSMD2))

Alternative Name: PSMD2

Background: PSMD2, TRAP2, MGC14274, S2, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor

Gene ID: 5708

NCBI Accession: NP_002799, NP_001265637, NP_001265638

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway