Trafficking Protein, Kinesin Binding 1 (TRAK1) antibody

Antigen: Trafficking Protein, Kinesin Binding 1 (TRAK1)

Synonyms: OIP106, hyrt, AI413908, AI467545, mKIAA1042, 2310001H13Rik, RGD1307844

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185333

Additional Information

Alternative name: OIP106 / TRAK1

Gene ID: 22906

Immunogen: CGAKLSKQTSLR

Description: All names and symbols: OIP106, OGT(O-Glc-NAc transferase)-interacting protein 106 KDa. Official Symbol: TRAK1. RefSeq number(s): NP_055780. Gene ontology terms: O-linked glycosylation, nucleus, protein binding, protein targeting, regulation of transcription from Pol II promoter

Specificity: Human, Chimp

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in Human Heart and Human Lung lysates (calculated MW of 77.3kDa according to NP_055780). Recommended concentration: 0.3-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185333 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Iyer, Akimoto, Hart: "Identification and cloning of a novel family of coiled-coil domain proteins that interact with O-GlcNAc transferase." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 5399-409, 2003 (PubMed).

Brickley, Smith, Beck et al.: "GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14723-32, 2005 (PubMed).