AKAP9 antibody (C-Term)

Catalog No. ABIN185354

The Goat Polyclonal anti-AKAP9 antibody has been validated for ELISA, WB and IF. It is suitable to detect AKAP9 in samples from Human.

Quick Overview for AKAP9 antibody (C-Term) (ABIN185354)

Target: AKAP9 (A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP9 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Grade: Verified

Product Details anti-AKAP9 Antibody

Binding Specificity: C-Term

Purpose: AKAP9 / AKAP450 / CG-NAP

Sequence: SGSTTQFHAG MR

Specificity: This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4, NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4, NP_671714.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: This product has been successfully used by a customer, showing a band at approx 450 kDa in lysates of cell line HepG2 (calculated MW of 453 kDa according to NP_005742.4). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKAP9

Target: AKAP9 (A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))

Alternative Name: AKAP9

Background: AKAP9, CG-NAP, PRKA9, YOTIAO, AKAP350, HYPERION, KIAA0803, A kinase (PRKA) anchor protein (yotiao) 9, AKAP120-like protein, kinase N-associated protein, A-kinase anchoring protein 450, A-kinase anchor protein, 350 kDa, protein kinase A anchoring protein 9

Gene ID: 10142

NCBI Accession: NP_005742, NP_671714

Pathways: M Phase, SARS-CoV-2 Protein Interactome