Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C) antibody

Antigen: Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C)

Synonyms: ERF1, TFAP2G, hAP-2g, AP2-GAMMA, Stra2, Ap-2.2, Tfap2c, AA409384, AP2gamma, AP2-gamma, erf1, ap-2g, tfap2g, MGC68493, ap2-gamma, tfap2gamma, TFAP2C, zgc:92088, hap-2g, tfa2pc, MGC143174

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185355

Additional Information

Alternative name: AP-2 gamma

Gene ID: 7022

Immunogen: Peptide with sequence C-NKTLEKMEKHRK, from the C Terminus of the protein sequence according to NP_003213.1

Format: Affinity-purified

Description: All names and symbols: TFAP2C, ERF1, TFAP2G, hAP-2g, AP2-GAMMA, transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma), estrogen receptor factor 1, transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma). Official Symbol: TFAP2C. RefSeq number(s): NP_003213.1. Gene ontology terms: cell-cell signaling, nucleus, regulation of transcription from Pol II promoter, transcription factor activity.

Specificity: Expected from sequence similarity: Human, Rat, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Hoei-Hansen, Rajpert-De Meyts, Carlsen et al.: "A subfertile patient diagnosed with testicular carcinoma in situ by immunocytological staining for AP-2gamma in semen samples: case report." in: Human reproduction (Oxford, England), Vol. 20, Issue 3, pp. 579-82, 2005 (PubMed).