Nanos Homolog 1 (NANOS1) antibody

Antigen: Nanos Homolog 1 (NANOS1)

Synonyms: NOS1, nanos1, fb76c12, wu:fb93h11, wu:fb98e07, wu:fq21a10, Xcat-2, nos1, xnos1, xcat-2, NANOS1, LOC100144352, NANOS1A

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185370

Additional Information

Alternative name: NANOS1

Gene ID: 340719

Immunogen: C-SARDGPPGKKLR

Format: Affinity-purified

Description: All names and symbols: NANOS1, nanos homolog 1 (Drosophila), NOS1. Official Symbol: NANOS1. RefSeq number(s): NP_955631.1

Specificity: This antibody is expected to recognise the longer isoform 1 only (NP_955631), ie not isoform 2 (NP_001009553). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1,13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185370 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Jaruzelska, Kotecki, Kusz et al.: "Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells." in: Development genes and evolution, Vol. 213, Issue 3, pp. 120-6, 2003 (PubMed).

Clark, Bodnar, Fox et al.: "Spontaneous differentiation of germ cells from human embryonic stem cells in vitro." in: Human molecular genetics, Vol. 13, Issue 7, pp. 727-39, 2004 (PubMed).