serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2) antibody

Antigen: serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2)

Synonyms: MEPI, PI14, PANCPIN, TSA2004, Spi14, 1810006A24Rik, MGC157389

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185374

Additional Information

Alternative name: MEPI / SERPINI2

Gene ID: 5276, 67931 (mouse), 171149 (rat)

Immunogen: C-KGDWKQKFRKEDTQ

Format: Affinity-purified

Description: All names and symbols: SERPINI2, MEPI, PI14, PANCPIN, TSA2004, SERPIN12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2. Official Symbol: SERPINI2. RefSeq number(s): NP_006208.1, NP_001012303.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_006208.1, NP_001012303.1). Human, Mouse, Rat, Dog, Chimpanzee

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185374 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185374 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining

Publications

Publications: Ozaki, Nagata, Suzuki et al.: "Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells." in: Genes, chromosomes & cancer, Vol. 22, Issue 3, pp. 179-85, 1998 (PubMed).

Xiao, Liu, Gentz et al.: "Suppression of breast cancer growth and metastasis by a serpin myoepithelium-derived serine proteinase inhibitor expressed in the mammary myoepithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3700-5, 1999 (PubMed).

Wehr, Furth, Sangar et al.: "Analysis of the human pancreatic stellate cell secreted proteome." in: Pancreas, Vol. 40, Issue 4, pp. 557-66, 2011 (PubMed).