APOE antibody (C-Term)

Catalog No. ABIN185375

The Goat Polyclonal anti-APOE antibody has been validated for WB and ELISA. It is suitable to detect APOE in samples from Human.

Quick Overview for APOE antibody (C-Term) (ABIN185375)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-APOE Antibody

Binding Specificity: C-Term

Purpose: APOE

Sequence: VGTSAAPVPS DNH

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VGTSAAPVPSDNH, from the C Terminus of the protein sequence according to NP_000032.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 36 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 36.2 kDa according to NP_000032.1). Recommended concentration: 0.05-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE

Background: APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset), LPG

Gene ID: 348

NCBI Accession: NP_000032

Pathways: Regulation of Cell Size, Lipid Metabolism