Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) antibody

Antigen: Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B)

Synonyms: CD32, FCG2, CD32B, FCGR2, IGFR2, FCGR2B, MGC137852

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185377

Additional Information

Alternative name: CD32 / FCGR2B

Gene ID: 2213

Immunogen: C-PDALEEPDDQNRI

Format: Affinity-purified

Description: All names and symbols: FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of IgG, low affinity IIb, receptor for (CD32), Fc gamma RIIb, Fc fragment of IgG, low affinity II, receptor for (CD32). Official Symbol: FCGR2B. RefSeq number(s): NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1,. Gene ontology terms: IgG binding, immune response, integral to membrane, plasma membrane, receptor activity, signal transduction

Specificity: This antibody is expected to recognise all four reported isoforms (NP_001002273, NP_001002274, NP_001002275, NP_003992) Human 310 aa, 290 aa, 291 aa, 309 aa,

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0kDa according to NP_003992.3,). In transfected HEK293 transiently expressing CD32 a band of approx. 40kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185377 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CD32 (RC211982) and probed with ABIN185377 (mock transfection in first lane), tested by Origene.

Publications

Publications: Hitomi, Tsuchiya, Kawasaki et al.: "CD72 polymorphisms associated with alternative splicing modify susceptibility to human systemic lupus erythematosus through epistatic interaction with FCGR2B." in: Human molecular genetics, Vol. 13, Issue 23, pp. 2907-17, 2004 (PubMed).

McGaha, Sorrentino, Ravetch: "Restoration of tolerance in lupus by targeted inhibitory receptor expression." in: Science (New York, N.Y.), Vol. 307, Issue 5709, pp. 590-3, 2005 (PubMed).