Charged Multivesicular Body Protein 5 (CHMP5) antibody

Antigen: Charged Multivesicular Body Protein 5 (CHMP5)

Synonyms: Vps60, CGI-34, PNAS-2, C9orf83, HSPC177, SNF7DC2, AW545668, RP23-28I8.4, 2210412K09Rik, RGD1305968, vps60, cgi-34, pnas-2, hspc177, MGC52936, MGC64125, zgc:64125, chmp5, MGC80100, MGC128530, MGC107865, DKFZp469M138

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185381

Additional Information

Alternative name: CHMP5

Gene ID: 51510

Immunogen: C-NKDGVLVDEFGLPQ

Format: Affinity-purified

Description: All names and symbols: CHMP5, chromatin modifying protein 5 , HGNC:26942, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, SNF7 domain containing 2, chromosome 9 open reading frame 83. Official Symbol: CHMP5. RefSeq number(s): NP_057494.2. Gene ontology terms: HIV infection, MVB sorting

Specificity: Human 219 aa

Synonyms: Vps60, CGI-34, PNAS-2, C9orf83, HSPC177, SNF7DC2, AW545668, RP23-28I8.4, 2210412K09Rik, RGD1305968, vps60, cgi-34, pnas-2, hspc177, MGC52936, MGC64125, zgc:64125, chmp5, MGC80100, MGC128530, MGC107865, DKFZp469M138

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18,280(11): 10548-55., PMID: 15644320). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185381 (xµg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ward, Vaughn, Shiflett et al.: "The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10548-55, 2005 (PubMed).