Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) antibody

Antigen: Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A)

Synonyms: PR65A, MGC786, PR65, MGC95083, ppp2r1a, MGC53746, MGC76072, zgc:56296, wu:fa02h04, sis, ssv, c-sis, pdgf2, MGC89548, PPP2R1A, MGC128399, Ppp2r1a, DKFZp470L0914, RP-C, PP2Ac, PP2CA, PP2A, R75353, Pp2a1, PPP2CA

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185382

Additional Information

Alternative name: PP2A / PPP2R1A

Gene ID: 5518

Immunogen: C-KYFAQEALTVLSLA

Format: Affinity-purified

Description: All names and symbols: PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform. Official Symbol: PPP2R1A. RefSeq number(s): NP_055040.2. Gene ontology terms: RNA splicing, antigen binding, binding, ceramide metabolism, cytosol, inactivation of MAPK, induction of apoptosis, membrane, microtubule cytoskeleton, mitochondrion, negative regulation of cell growth, negative regulation of tyrosine phosphorylation of Stat3 protein, nucleus, phosphoprotein phosphatase activity, protein amino acid dephosphorylation, protein binding, protein complex assembly, protein heterodimerization activity, protein phosphatase type 2A activity, protein phosphatase type 2A complex, protein phosphatase type 2A regulator activity, regulation of DNA replication, regulation of Wnt receptor signaling pathway, regulation of cell adhesion, regulation of cell cycle, regulation of cell differentiation, regulation of growth, regulation of transcription, regulation of translation, response to organic substance, second-messenger-mediated signaling, soluble fraction

Specificity: Human, Mouse, Rat, Dog 589 aa

Synonyms: PR65A, MGC786, PR65, MGC95083, ppp2r1a, MGC53746, MGC76072, zgc:56296, wu:fa02h04, sis, ssv, c-sis, pdgf2, MGC89548, PPP2R1A, MGC128399, Ppp2r1a, DKFZp470L0914, RP-C, PP2Ac, PP2CA, PP2A, R75353, Pp2a1, PPP2CA

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 60kDa band observed in Human Thymus and Lymph Node lysates (calculated MW of 65.3kDa according to NP_055040.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185382 (0.03µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hong, Sarge: "Regulation of protein phosphatase 2A activity by heat shock transcription factor 2." in: The Journal of biological chemistry, Vol. 274, Issue 19, pp. 12967-70, 1999 (PubMed).

Takakura, Kohno, Manda et al.: "Genetic alterations and expression of the protein phosphatase 1 genes in human cancers." in: International journal of oncology, Vol. 18, Issue 4, pp. 817-24, 2001 (PubMed).