RPTOR Independent Companion of MTOR, Complex 2 (RICTOR) antibody

Antigen: RPTOR Independent Companion of MTOR, Complex 2 (RICTOR)

Synonyms: AVO3, AW492497, 4921505C17Rik, 6030405M08Rik, D530039E11Rik, PIA, mAVO3, KIAA1999, MGC39830, DKFZp686B11164, Fyb, RICTOR

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185390

Additional Information

Alternative name: RICTOR

Gene ID: 253260, 78757 (mouse)

Immunogen: C-KQPIVDTSAES

Description: All names and symbols: Rictor, KIAA1999, AVO3, MGC39830, likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae), rapamycin-insensitive companion of mTOR, mAVO3, pianissimo, DKFZp686B11164. Official Symbol: RICTOR. RefSeq number(s): NP_689969.2

Specificity: Human, Mouse, Dog 1708

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185390 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Sarbassov, Ali, Kim et al.: "Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton." in: Current biology : CB, Vol. 14, Issue 14, pp. 1296-302, 2004 (PubMed).

Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).