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Antibodies
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anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF...
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) antibody
| Antigen |
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| Synonyms |
GIF, GLIF, MMIF, Glif, MGC107654, MGC72801, MIF, MGC127044, mif, Mif, gif, glif, mmif, LOC100136498, LOC100284350, LOC100284546 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Enzyme Immunoassay (EIA)
Western Blotting (WB) (34), ELISA (22), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (7), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN185406 |
| Quantity |
100µg (100µg/200µl) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MIF
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Gene ID
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4282
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Swiss-Prot
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NP_002406.1
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Immunogen
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Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.
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Cross-Reactivity
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Human, Rat (Rattus), Mouse (Murine)
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Format
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Liquid
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Grade
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Serum-free
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Description
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Official Symbol: MIF
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Specificity
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Human, Rat, Mouse
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 13kDa band observed in Human Lymph Node and Thymus lysates (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
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Concentration
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100µg/200µl
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Immunology, Innate Immunity, Cytokines, Inflammation
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Restrictions
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For Research Use only
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Publications
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Church, Willis, Priest et al.: "Obesity, macrophage migration inhibitory factor, and weight loss." in: International journal of obesity (2005), Vol. 29, Issue 6, pp. 675-81, 2005 (PubMed).
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Alternatives for antigen "Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF)", type "Antibodies"
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Hosts
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Rabbit (29), Mouse (18), Goat (4), Chicken (2)
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Reactivities
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Human (46), Rat (Rattus) (22), Mouse (Murine) (19), Cow (Bovine) (16), Dog (Canine) (16), Pig (Porcine) (15), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (34), ELISA (22), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (7), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Biotin (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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