Cholecystokinin B Receptor (CCKBR) antibody

Antigen: Cholecystokinin B Receptor (CCKBR)

Synonyms: CCKBR, MGC137600, CCK-CHR, DKFZp459M1310, LOC100232835, CCK-B, CCK-BR, CCK2-R, CCK2R, CCKR-2, Cck2r, Cholrec

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185407

Additional Information

Alternative name: CCKBR

Gene ID: 887, 12426 (mouse), 25706 (rat)

Immunogen: C-FDGDSDSDSQSRVRNQ

Description: All names and symbols: CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor. Official Symbol: CCKBR. RefSeq number(s): NP_795344.1

Specificity: Human, Mouse, Cow 447 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data kindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by International Journal of Endocrinology (in press). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov,51(11):1501-13, PMID: 14566022). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology, Endocrine system

Restrictions: For Research Use only

Images

ABIN185407 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Olszewska-Pazdrak, Townsend, Hellmich: "Agonist-independent activation of Src tyrosine kinase by a cholecystokinin-2 (CCK2) receptor splice variant." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40400-4, 2004 (PubMed).

Product: El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, 2010 (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, 2009 (PubMed).

Berna, Seiz, Nast et al.: "CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38905-14, 2010 (PubMed).

Quattrone, Dewaele, Wozniak et al.: "Promoting role of cholecystokinin 2 receptor (CCK2R) in gastrointestinal stromal tumour pathogenesis." in: The Journal of pathology, 2012 (PubMed).