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Antibodies
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anti-Pancreatic Prohormone (PPY) antibody
Pancreatic Prohormone (PPY) antibody
| Antigen |
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| Synonyms |
PNP |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN185420 |
| Quantity |
100 ug (0.5 mg/ml) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Pancreatic Polypeptide / PPY
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Gene ID
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5539
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Immunogen
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Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
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Format
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Affinity-purified
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Description
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All names and symbols: PPY, pancreatic polypeptide , HGNC:9327, PNP. Official Symbol: PPY. RefSeq number(s): NP_002713.1
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Specificity
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Expected from sequence similarity: Mouse, Rat, Dog
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 35-38kDa band in human pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration, 3µg/ml.
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Concentration
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0.5 mg/ml
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Badman, Flier: "The gut and energy balance: visceral allies in the obesity wars." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1909-14, 2005 (PubMed).
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Alternatives for antigen "Pancreatic Prohormone (PPY)", type "Antibodies"
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Hosts
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Rabbit (12), Goat (4), Mouse (1)
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Reactivities
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Human (11), Cow (Bovine) (3), Dog (Canine) (3), Mouse (Murine) (3), Rat (Rattus) (3), Hamster (1), Pig (Porcine) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (4), Enzyme Immunoassay (EIA) (3), Western Blotting (WB) (3), Immunofluorescence (IF) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1)
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