Caveolin 1, Caveolae Protein, 22kDa (CAV1) antibody

Antigen: Caveolin 1, Caveolae Protein, 22kDa (CAV1)

Synonyms: CAV, CGL3, BSCL3, VIP21, MSTP085, Cav, Cav-1, MGC187299, CAV1, fc07c04, wu:fc07c04, cav1a, cav1b, caveolin 1, cav, vip21, mstp085, MGC107940, Caveolin-1, cav1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185421

Additional Information

Alternative name: Caveolin 1

Gene ID: 857, 12389 (mouse), 25404 (rat)

Immunogen: C-DELSEKQVYDAH

Format: Affinity-purified

Description: All names and symbols: CAV1, caveolin 1, caveolae protein, 22kDa , HGNC:1527, CAV, MSTP085, VIP21 , caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32. Official Symbol: CAV1. RefSeq number(s): NP_001744.2

Specificity: No cross-reactivity expected to Caveolin 2 and Caveolin 3 Human, Mouse, Rat, Dog, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 21kDa and 25kDa observed in Human Heart, Lung and Spleen lysates. These bands correspond to earlier findings in literature with different antibodies (Wiechen et al Am J Pathol. 2001 Mar,158(3):833-9, PMID: 11238032 ). In transfected HEK293 transiently expressing Caveolin 1 a band of approx. 23kDa is observed. This band is not observed in the non-transfected HEK293. This protein has a calculated MW of 20.5kDa according to NP_001744.2. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Organelles, Signaling

Restrictions: For Research Use only

Images

ABIN185421 (0.3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Caveolin 1 (RC210274) and probed with ABIN185421 (mock transfection in second lane), tested by Origene

Publications

Publications: Cavallo-Medved, Mai, Dosescu et al.: "Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells." in: Journal of cell science, Vol. 118, Issue Pt 7, pp. 1493-503, 2005 (PubMed).