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Antibodies
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anti-Spleen tyrosine Kinase (SYK) antibody
Spleen tyrosine Kinase (SYK) antibody
| Antigen |
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| Synonyms |
FLJ25043, FLJ37489, DKFZp313N1010, Syk, p72syk |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (68), Immunofluorescence (IF) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (25), Flow Cytometry (FACS) (18), ELISA (14), Enzyme Immunoassay (EIA) (13), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN185423 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Spleen tyrosine kinase / SYK
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Gene ID
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6850, 20963 (mouse), 25155 (rat)
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Immunogen
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C-KYLQQNRHVKDKN
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Format
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Affinity-purified
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Description
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All names and symbols: SYK, spleen tyrosine kinase, HGNC:11491. Official Symbol: SYK. RefSeq number(s): NP_003168.2
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Specificity
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Human, Mouse, Rat, Dog
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1kDa according to NP_003168.2). Recommended concentration: 1-3µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Rogers, Slack, Edwards et al.: "Syk-dependent cytokine induction by Dectin-1 reveals a novel pattern recognition pathway for C type lectins." in: Immunity, Vol. 22, Issue 4, pp. 507-17, 2005 (PubMed).
Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).
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Alternatives for antigen "Spleen tyrosine Kinase (SYK)", type "Antibodies"
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Hosts
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Rabbit (71), Mouse (24), Goat (4)
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Reactivities
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Human (90), Mouse (Murine) (30), Rat (Rattus) (28), Dog (Canine) (19), Cow (Bovine) (18), Pig (Porcine) (16), Chicken (1)
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Applications
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Western Blotting (WB) (68), Immunofluorescence (IF) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (25), Flow Cytometry (FACS) (18), ELISA (14), Enzyme Immunoassay (EIA) (13), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pTyr323 (14), C-Term (2), Y525/526 (1), pTyr525, pTyr526 (1), pTyr525/526 (1)
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Alternatives 
