Nanog Homeobox (NANOG) antibody

Antigen: Nanog Homeobox (NANOG)

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN185444

Additional Information

Alternative name: NANOG

Gene ID: 79923, 71950 (mouse), 414065 (rat)

Immunogen: Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2

Description: All names and symbols: NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48. Official Symbol: NANOG. RefSeq number(s): NP_079141.2.

Specificity: Expected from sequence similarity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1µg/ml. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse. Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2,460(7251):53-9. Epub 2009 May 31. (PMID: 19483674).

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Hoei-Hansen, Almstrup, Nielsen et al.: "Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours." in: Histopathology, Vol. 47, Issue 1, pp. 48-56, 2005 (PubMed).

Hyslop, Stojkovic, Armstrong et al.: "Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 8, pp. 1035-43, 2005 (PubMed).

Skotheim, Lind, Monni et al.: "Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development." in: Cancer research, Vol. 65, Issue 13, pp. 5588-98, 2005 (PubMed).

Ralston, Rossant: "Genetic regulation of stem cell origins in the mouse embryo." in: Clinical genetics, Vol. 68, Issue 2, pp. 106-12, 2005 (PubMed).

Product: Raya, Rodríguez-Pizà, Guenechea et al.: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena et al.: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, 2010 (PubMed).

Montserrat, Garreta, González et al.: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, 2011 (PubMed).

Vassena, Montserrat, Carrasco Canal et al.: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed).