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Nanog antibody (N-Term)

NANOG Reactivity: Human, Mouse ChIP-seq, ChIP, CUT&RUN, ICC, IF, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6972403
$616.85
Plus shipping costs $45.00
100 μL
Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target See all Nanog (NANOG) Antibodies
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Binding Specificity
    • 16
    • 16
    • 14
    • 12
    • 11
    • 10
    • 10
    • 8
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 162
    • 59
    • 26
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 139
    • 19
    • 9
    • 1
    Rabbit
    Clonality
    • 140
    • 28
    Polyclonal
    Conjugate
    • 81
    • 12
    • 12
    • 11
    • 9
    • 7
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This Nanog antibody is un-conjugated
    Application
    • 104
    • 84
    • 57
    • 50
    • 32
    • 26
    • 26
    • 23
    • 19
    • 16
    • 8
    • 8
    • 7
    • 1
    • 1
    • 1
    ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
    Characteristics
    Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). Nanog antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
    Purification
    Affinity Purified
    Immunogen
    This antibody was raised against a peptide within the N-terminal region of mouse Nanog.
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Alternative Name
    Nanog (NANOG Products)
    Synonyms
    2410002E02Rik, ENK, ecat4, NANOG, hacp, wu:fd19e04, wu:fd20a07, zgc:193933, Nanog homeobox, nanog homeobox, Nanog, NANOG, nanog
    Background
    Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
    Molecular Weight
    45 kDa
    NCBI Accession
    NP_082292
    Pathways
    Stem Cell Maintenance
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