Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Nanog antibody (N-Term)

NANOG Reactivity: Human, Mouse WB, IF, ICC, ChIP-seq, ChIP, CUT&RUN Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6972403
  • Target See all Nanog (NANOG) Antibodies
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Binding Specificity
    • 16
    • 16
    • 12
    • 10
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 137
    • 58
    • 23
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 127
    • 15
    • 3
    • 1
    Rabbit
    Clonality
    • 122
    • 24
    Polyclonal
    Conjugate
    • 76
    • 10
    • 9
    • 9
    • 8
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This Nanog antibody is un-conjugated
    Application
    • 115
    • 65
    • 48
    • 46
    • 26
    • 26
    • 24
    • 18
    • 17
    • 17
    • 8
    • 7
    • 5
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Characteristics
    Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). Nanog antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
    Purification
    Affinity Purified
    Immunogen
    This antibody was raised against a peptide within the N-terminal region of mouse Nanog.
    Isotype
    IgG
    Top Product
    Discover our top product NANOG Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Alternative Name
    Nanog (NANOG Products)
    Synonyms
    2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
    Molecular Weight
    45 kDa
    NCBI Accession
    NP_082292
    Pathways
    Stem Cell Maintenance
You are here:
Support